现在有显微技术能观测活的细胞分化吗? 第1页

相衬显微技术于 1930 年代发明，相差显微镜在 1940 年代成功地对活细胞成像并拍成动态画面。发明者 Frits Zernike 于 1953 年获得诺贝尔物理学奖。

可以看看 1943 年的视频^[1]：

Georges Nomarski 于 1952 年发明的微分干涉相差显微镜、Robert Hoffman 于 1975 年发明的霍夫曼调制对比显微镜亦可对活细胞成像。

利用荧光蛋白、活细胞染料等标记细胞，可以在荧光显微镜下研究绝大部分细胞过程。

共聚焦反射显微镜可以对未标记的活组织成像，尤其适合研究眼睛。

参考

1. ^ Time lapse movie of the prophase stage during meiosis I in spermatocytes of the locust Psophus stridulus. This is a historic movie made in the early 40s by Kurt Michel of the ZEISS company in Jena using Zernike's newly invented phase contrast microscope. Several sequences are shown, some proceeding to metaphase. Despite its early date, the movie and the others in the group remain some of the clearest examples of chromosome behavior during meiosis. Based on 2hr real time duration. Originally published by Institut für den Wissenschaftlichen Film, Goettingen.